Science & Technology
We have conceived a microfluidic-based technology that transforms the surface of picoliter-size droplets into a highly sensitive sensors (down 10-21 mole sensibility) enabling to measure the molecules that are secreted by a single cell encapsulated into the droplet.
The resolvable signal generated allow to trigger sorting event and recover the droplets containing single cells showing secretory profile of interest. Rare sub-population (resolution 1/10.000) can be recovered and re-expended or summited to complementary analysis.
European Patent N° 16,305,394.5; PCT / EP / 2017/058035).
Based on a two-component molecular system and a so-called bio-orthogonal chemical reaction, the MicroOmiX technology allows direct use of the internal surface of the droplet for detection of biomarkers.
We have, so far, focused on a first application, antibody development. Our versatile technology can be implemented in different ways according to the sorting criteria affinity, selectivity or quantity.
The cell-in droplet can be kept alive for up to four days giving them time to secrete they signature biomarkers which are detected at droplet’s interface. On-line analysis and sorting based on characteristic fluorescence signal allows the recovery of selected sub populations.